Allows access to large-scale screening data (including but not limited to Tox21 qHTS data), along with the medicinal chemistry and informatics necessary to identify chemical probes to study the functions of genes, cells, and biochemical pathways. PubChem is organized as three linked databases within the NCBI's Entrez information retrieval system. These databases are PubChem Substance, PubChem Compound, and PubChem BioAssay.
Access chemical structure, annotations, and quality control information.
The EPA Computational Toxicology (CompTox) Chemicals Dashboard, formerly the Chemistry Dashboard, helps decision-makers and scientists quickly and efficiently evaluate thousands of chemicals. The Dashboard is a one-stop-shop for chemistry, toxicity and exposure information for over 760,000 chemicals. Data and models within the Dashboard also help with efforts to identify chemicals of most need of further testing and reducing the use of animals in chemical testing. The Tox21 chemicals list and associated data is available on the Dashboard.
Tox21 researchers work on a variety of different assays.
Tox21 currently is accepting assay proposals from investigators in all areas, both within and outside NIH and the federal government. Follow the instructions provided below to nominate an assay.
Proposed assays should be compatible with the qHTS guidelines in NCATS’ assay guidance criteria.
For questions regarding nominating an assay, please email: Menghang Xia, Ph.D 
.
[otw_shortcode_button size="medium" icon_position="left" shape="square" css_class="red" href="https://tools.niehs.nih.gov/webforms/index.cfm/main/formViewer/form_id/501"]Tox21 Assay Nomination Form[/otw_shortcode_button]
Nomination
Researchers from academia, private institutions and government/non-government organizations may nominate assays.
Review
The Tox21 Pathways/Assays Working Group (with representatives from each of the Tox21 partners) reviews submitted assays on an ongoing basis. Tox21 leadership provides final approval. Assays are selected based on their biological and toxicological relevance and adaptability to miniaturization and qHTS screening.
Optimization and Miniaturization
Tox21 staff optimize and miniaturize selected assays into the qHTS screening platform.
Validation and Screening
In robotic online validation, the assay is screened in triplicate against a validation library. Tox21 experts evaluate validation data for reproducibility and positive control consistency before submission to the Pathways/Assays Working Group for approval.
Data Analysis and Dissemination
Tox21 staff analyze the resulting information and send raw data and/or summary tables to Tox21 partners on a regular basis. All results are made publicly available.
Allows access to large-scale screening data (including but not limited to Tox21 qHTS data), along with the medicinal chemistry and informatics necessary to identify chemical probes to study the functions of genes, cells, and biochemical pathways. PubChem is organized as three linked databases within the NCBI's Entrez information retrieval system. These databases are PubChem Substance, PubChem Compound, and PubChem BioAssay.
Visualization of Tox21 qHTS data including concentration-response curves, curve fitting results and different activity metrics along with chemical structure and analytical QC results. Data are searchable by assay and/or chemical. Results from multiple assays and/or chemicals can be overlaid for ease of comparison. All data as well as assay descriptions and detailed screening protocols are available for download.
A collection of flat files for Tox21 and ToxCast programs. Files include assay and chemical descriptions, HTS data, and other related files.
Browse pathways by source, species, biological function/process, disease/toxicity relevance and availability of probing assays. Pathway enrichment analysis of gene sets. This integrated platform will facilitate systematic analysis and modeling of toxicity responses.
The EPA Computational Toxicology (CompTox) Chemicals Dashboard, formerly the Chemistry Dashboard, helps decision-makers and scientists quickly and efficiently evaluate thousands of chemicals. The Dashboard is a one-stop-shop for chemistry, toxicity and exposure information for over 760,000 chemicals. Data and models within the Dashboard also help with efforts to identify chemicals of most need of further testing and reducing the use of animals in chemical testing. The CompTox Chemicals Dashboard integrates data from various sources including:
The Tox21 toolbox contains useful tools for accessing and visualizing the Tox21 quantitative high throughput screening (qHTS) 10K library data, as well as integrating with other publicly available data.
